Write out the equation that describes the mechanism for enzyme action used as a model by Michaelis and Menten. List the important assumptions used by Michaelis and Menten to derive a rate equation for this reaction. 84. An enzyme catalyzes the reaction A - B. The initial rate of the reaction was measured as a function of the concentration of A. The following data were obtained: (A), micromolar V nmoles/min 0.08 0.05 0.5 100 500 1,000 5,000 10,000 20,000 a) b) What is the K. of the enzyme for the substrate A? What is the value of V, when (A) = 43? The above data were plotted as 1/V, versus 1[A], and a straight line was obtained. c) d) What is the value of the y-intercept of the line? What is the value of the x-intercept of the line?