Why did the authors examine the effects of double-stranded RNA on transgenic C. elegans expressing two different green fluorescent (GFP) proteins?
* What clues did the studies provide about the possible mechanism of double-stranded RNA interference?
* As Fire et al. predicted in this 1998 paper, interference by double-stranded RNAs has now gone on to be used to eliminate or reduce expression in other invertebrates, in vertebrates, and in plants. However, the authors cautioned about possible limitations. What are these?