After streak and stab inoculating an unknown bacterium in a tube of KIA medium on a Thursday you incubate it for 24hrs at 37C. You peek at the tube on Friday and find that it is yellow/yellow with cracking of the agar. You continue to incubate the tube over the weekend so you can take images of it on Monday. When you grab the tube Monday morning it now looks Red/Red with large bubbles. Should Fridays results or Mondays results be considered most accurate? Why do you think it changed? After streak and stab inoculating an unknown bacterium in a tube of KIA medium on a Thursday you incubate it for 24hrs at 37C. You peek at the tube on Friday and find that it is yellow/yellow with cracking of the agar. You continue to incubate the tube over the weekend so you can take images of it on Monday. When you grab the tube Monday morning it now looks Red/Red with large bubbles. Should Fridays results or Mondays results be considered most accurate? Why do you think it changed? Mondays results, the final result after the incubations have taken place is most accurate. The yellow/yellow result was too early so the tube hadn't had sufficient time to change color. Monday's results, the tube was likely inoculated incorrectly and thus it took time for the motile organisms to move from the slant to the butt of the tube. Friday's results, after 24hrs the unknown organism had sufficient time to illustrate its metabolic capabilities. The longer incubation caused the organism to run out of lactose thus requiring protein to be used. Friday's results, after incubating the tube for 3 days the acids turned into alkaline products due to death of the organisms. None of the results should be considered accurate; over the weekend the tube must have been contaminated