The steps involved in the Southern Blotting should be performed in what order? 1.exposure of x-ray film to membrane 2.immobilization of ssDNA onto membrane 3.hybridization with radioactive probe 4.DNA electrophoresis 5.DNA digestion with restriction enzyme
a.5, 2, 4, 3, 1
b.5, 4, 3, 2, 1
c.3, 2, 5, 4, 1
d.5, 4, 2, 3, 1