2 ng of a 2500 base pairs double strand DNA is obtained from a National Genetic laboratory in Ghana. The purpose is to amplify the DNA using recombinant techniques.
A. What is a recombinant DNA?
B. In addition to the DNA provided, what other DNAs and enzymes are needed to produce a recombinant DNA. Explain their role in designing the recombinant DNA.
C. If the 2500 base pairs DNA contained 27% cytosines, calculate the percentage of guanines, thymines and adenines.
D. After sequencing, you realised that 4 adenines of the 2500 double stranded DNA were mutated to cytosines, calculate the percentage of adenines, thymines, cytosines and guanines.