What is the molecular basis for the difference in the electrophoretic mobility of the protein Cytochrome C analyzed in the electrophoresis exercise? Relate your answer to the isoelectric point of Cytochrome C, which is 10.2. Also, the buffer used during gel electrophoresis was Tris Glycine, which had a pH 8.6. NOTE: Your answer should only pertain to Cytochrome C, and not the other proteins, as you will be penalized for doing so.
a) Cytochrome C will move towards the anode because the pH of the buffer is below its isoelectric point.
b) Cytochrome C will move towards the cathode because the pH of the buffer is above its isoelectric point.
c) Cytochrome C will not move as its isoelectric point matches the pH of the buffer.
d) Cytochrome C will move towards the anode because the pH of the buffer is higher than its isoelectric point.