G you extracted your dna from cheek cells. the concentration of your dna sample is 250 ng/μl. the dna template for pcr should be 10 ng/μl. you should mix _ μl of your dna sample and _ μl of water to make final volume of 100 μl of dna that has the concentration of 10 ng/μl.

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22-06-2018
Biology

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G you extracted your dna from cheek cells. the concentration of your dna sample is 250 ng/μl. the dna template for pcr should be 10 ng/μl. you should mix _ μl of your dna sample and _ μl of water to make final volume of 100 μl of dna that has the concentration of 10 ng/μl.

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gradman555p98x9p gradman555p98x9p · Answer 1 · 2018-07-02T00:40:31+02:00

You should mix 4µl of DNA sample and complete it with 96µl (100-4) of water.

You can figure this out by using C1.V1 = C2.V2
C1 = 250 ng/μl (Sample Concentration)
V1 = ? (the necessary volume to take from the DNA sample)
C2 = 10 ng/μl (PCR concentration)
V2 = 100 µl (total volume)

V1 = [tex] \frac{C2 . V2}{C1} [/tex] = [tex] \frac{10 . 100}{250} [/tex] = 4µl

For the water: 100µl (total volume) = 4µl + Water
Water volume = 96µl

G you extracted your dna from cheek cells. the concentration of your dna sample is 250 ng/μl. the dna template for pcr should be 10 ng/μl. you should mix _____ μl of your dna sample and _____ μl of water to make final volume of 100 μl of dna that has the concentration of 10 ng/μl.

Respuesta :

Otras preguntas

G you extracted your dna from cheek cells. the concentration of your dna sample is 250 ng/μl. the dna template for pcr should be 10 ng/μl. you should mix _ μl of your dna sample and _ μl of water to make final volume of 100 μl of dna that has the concentration of 10 ng/μl.