Solution:
DF = Initial dilution * Subsequent dilution * Amount Plated
Total Count Per g = [tex]\frac{1}{DF}[/tex] * Colonies Counted
Initial dilution : [tex]\frac{43}{(43+350)}=\frac{43}{393}[/tex]
Subsequent dilution : [tex](\frac{1}{6} )*(\frac{1}{90} )*(\frac{1}{61} )[/tex]
Amount Plated : 0.1
Colonies Counted : 143
DF = [tex]\frac{43}{393}*\frac{1}{6}*\frac{1}{90}*\frac{1}{61}*0.1[/tex]
DF=[tex]\frac{43}{393}*\frac{1}{6}*\frac{1}{90}*\frac{1}{61}*\frac{1}{10}[/tex]
DF = [tex]\frac{43}{129454200}[/tex]
Total Count Per g = [tex](\frac{129454200}{43} )*143[/tex]
Total Count Per g = [tex]4.3*10^{8}[/tex]
