Eukaryotic Initiation:
1) ____ and ____ bind to the small and large ribosomal subunits respectively to prevent them from ______ without mRNA
2) _______ complex is formed.
3) _______ complex is formed.
4) Cells can regulate protein synthesis by _____ a ____ residue on the ____ bound to GDP. The phosphorylated complex is unable to exchange the bound GDP for GTP and cannot bind _____, thus inhibiting protein synthesis.
5) As the mature mRNA is transported from the nucleus into the cytoplasm, initiation factors (____) for protein synthesis binds to the ____.
6) The ____ binds to the ____ to form the ____ through an interaction of the ____ subunit and ____. The initiation complex then ____ along the mRNA to look for the Kozak sequence containing the first AUG.
7) The helicase ____, which is activated by ____, uses ATP to unwind RNA structures.
8) Scanning ____ as the met-tRNAimet anticodon _____ the AUG codon (Kozack sequence).
9) ____ hydrolyzes to eIF2-GDP, an irreversible step that prevents further scanning.
10) The eIFs ____.
11) ____ helps bring in ____; GTP is hydrolized and the IFs are released.
12) Met- tRNAimet positioned at the _ site.