Temperature is a factor considered when a cell cultures environment is made as similar as possible to a cells natural surroundings
The propagation of cells outside the organism of origin is called cell culture, these techniques allow the maintenance of cells 'in vitro', maintaining their properties to the maximum under controlled conditions.
The environment of the cell culture can be influenced by the nature of the substrate (solid, semi-solid or liquid), the contact with other cells, the constitution of the medium and the temperature.
In a cell culture, the physical-chemical conditions (pH, temperature, osmotic pressure, partial pressure of O2 and CO2), and physiological conditions (hormones, growth factors, etc.) can be controlled.
To establish a defined environment, it is necessary to know precisely the nutritional needs of the cells in question.
- The optimal temperature for cell culture depends mainly on the body temperature of the organism from which the cells were obtained, to obtain reproducible results, consistency in temperature must be ensured.
- Temperature also influences the pH of the culture medium, because at low temperatures CO2 dissolves more.
Therefore, we can conclude that temperature considered when a cell cultures environment is made as similar as possible to a cells natural surroundings.
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