Answer:
DNA ligase
Explanation:
DNA ligase is an enzyme that catalyzes the formation of a phosphodiester bonds. It is active during DNA replication where it connects DNA fragments into continuous strand. It is also involved in repair of single-strand and double-strand breaks.
DNA ligase enzyme is widely used in molecular biology laboratories for recombination of DNA: in gene cloning to join DNA molecules together to form recombinant DNA.