Answer: Two tRNA should be placed on the ribosome P and A binding sites. The tRNA who has an attached codon corresponding to the mRNA codon above P site should be dragged on P site, and the tRNA who has an attached codon corresponding to the mRNA codon above A site should be dragged on A binding site. E binding site on the ribosome should be left empty.
Explanation:
The large ribosome unit in translation has 3 binding sites:
1. E binding site (Exit site) which is empty after an amino acid bond is formed (Because after it is formed, a previous tRNA had to leave this Exit site)
2. P binding site (Polypeptide site) which is the place in which the aminoacid bond is formed.
3. A binding site (Aminoacid site) in which new amino acids are welcome according to the mRNA lead, to build another peptide bond.
All tRNA passes through all these sites but immediately a new peptide bond forms we have the P and A sites occupied. Then they will shift to the left and one of the tRNA will leave after being in E site, and the other will remain on the P site, waiting for another peptide bond to be formed.