In fruit flies, red eyes (pr+_) are dominant to purple eyes (prp) and normal wings (vg+_) are dominant to vestigial wings (vgv). The genes are located on the same chromosome. A pure-breeding red-eyed fly with vestigial wings was crossed with a pure-breeding purple-eyed fly with normal wings. All of the F1 progeny had a WT phenotype. The recombination frequency between the two genes is 15%. If an F1 individual were test crossed, what percentage of the progeny would you expect to have the WT phenotype?

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Answer:

7.5%

Explanation:

The genes vg+/vg and pr+/pr are linked.

A pure-breeding red-eyed fly with vestigial wings was crossed with a pure-breeding purple-eyed fly with normal wings.

This cross can be written down as:

[tex]\frac{pr+ \ vg }{pr+ \ vg}[/tex] x [tex]\frac{pr \ vg+ }{pr \ vg+}[/tex]

The wild-type F1 would be:

[tex]\frac{pr+ \ vg}{pr \ vg+}[/tex]

They are test crossed with a homozygous recessive individual that can produce only one type of gamete: pr vg

The F1 flies will produce 4 types of gametes:

  • pr+  vg , parental
  • pr  vg+ , parental
  • pr+ vg+ , recombinant
  • pr  vg , recombinant

Each time recombination happens, the two possible recombinant gametes are generated. Because the recombination frequency between the two genes is 15% altogether, each recombinant gamete will appear with half that frequency, 7.5% of the times.

The pr+ vg+ recombinant gamete will be produced with a frequency of 7.5%, and when it's combined with the pr vg gamete from the test cross individual, it will give rise to offpring with WT phenotype.

The expected percentage of the progeny to have the WT phenotype is 5 %

What is progeny?

Progeny means the next generation, it means the offspring or children.

Parental

[tex]\rm \dfrac{Pr^+Vg}{Pr^+Vg} \times \dfrac{PrVg^+}{PrVg^+}[/tex]

F1 generation

[tex]\rm \dfrac{Pr^+Vg}{Pr^+Vg} \times \dfrac{PrVg^+}{PrVg^+}[/tex]

Cross over

Red eye vestigial wings

[tex]\rm \dfrac{ Pr^+Vg}{PrVg}[/tex]

Purple eye Normal wings

[tex]\rm \dfrac{ PrVg}{PrVg}[/tex]

Recombination at the F1 genotype from the parent to

[tex]\dfrac{Pr^+Vg^+}{PrVg} = 10\%[/tex]

Test Cross for the recombination:

[tex]\rm \dfrac{Pr^+Vg}{PrVg} \times \dfrac{PrVg}{PrVg}[/tex]

F1

Red eye vestigial wings

[tex]\rm \dfrac{ Pr^+Vg}{PrVg}[/tex]

Purple eye Normal wings

[tex]\rm \dfrac{ PrVg}{PrVg}[/tex]

Thus, the percentage will be 5%

Learn more about progeny

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