Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site, this enzyme-inhibitor bond is not cleaved by the enzyme. This is an example of an Irreversible kind of inhibition.
Explanation:
- Cell Lysates are prepared by using Phenyl-methane-sulfonyl-fluoride (PMSF). This PMSF is an enzyme inhibitor that inactivates serine proteases.
- It inactivate the serine proteases by attaching with the catalytic serine, Which was in an active state.
- The PMSF bond was not separated by the enzyme. So this action cannot be repeated. Hence it is an irreversible kind of inhibition.
- Handling of PMSF should be done in a fume hood and while handling it we should wear gloves.
