Which of the following statements is/are not true about bacterial transformation?

a)Cells most often can only be transformed when in the competent state.

b)Transformation may only be performed using plasmid DNA containing antibiotic-resistance genes (R factors).

c)Transformed cells are capable of passing their newly acquired traits onto succeeding generations.

d) E.coli is not naturally competent it must first be treated artificially in the laboratory before they are capable of undergoing transformation.

e)The "+" and "-" means that the bacteria was ampicillin resistant and ampicillin non resistant respectively.

f)Bacteria are selective in what DNA they find and incorporate from their environment by transformation.

Respuesta :

B

Transformation of  plasmids with antibiotic-resistant genes (in addition to desired genes to be transformed) is done in the lab mainly as a tool of artificial selection to eliminate bacteria that did not take up the exogenous DNA.

Explanation:

Transformation must occur in competent cells which can be artificially induced by culturing the cells in divalent cations like calcium chloride, or by electroporation. Nonetheless, cells can take up any exogenous DNA when they become competent. The exogenous DNA after being taken up is involved in recombination with the cell's DNA. This ensures that the exogenous DNA is incorporated into the genome of the cell hence exhibiting the new genes in phenotype. This is one method of creating transgenic organisms.

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