Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
The configuration that should might the ligated plasmids have is Nicked circle
At the time of DNA technology, the gene of interest should be ligated to plasmid DNA via excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase. Enzyme ligase catalyzed via ATP seals the nicks by preparing the phosphodiester bonds in the ds or ss DNA and release of ADP. Also, The ligation in the plasmid did not arise properly.
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