Respuesta :
Answer:
Extension
Explanation:
Polymerase chain reaction is given by Kary Mullis and it contains three steps denaturation, annealing, and extension. In denaturation, the temperature is raised to 96° c so that the DNA can denature.
In annealing, the temperature gets reduced to 55°c so that the primer can bind to the complementary sequence. In extension, Taq polymerase is used to extend the new strand.
This enzyme is isolated from thermophilic bacteria and the temperature here is raised to 72°c so that the enzyme can extend the primer and make the complementary strand. So the right answer is extension.
Polymerase Chain Reaction (PCR) is a technique that is utilized to produce thousands of copies of gene segments identical to the targeted gene segment.
The step of the protocol that makes the heat-stability most necessary is the third step (extension).
- There are three major steps to PCR protocol. This includes Denaturation which involves a high heat at 96-98 degree Celsius to unwound the DNA helix.
- Then, annealing, where short DNA molecules called primers bind to flanking regions of the target DNA. Here, the temperature is reduced down to 53-55.
- Then, extension, in which DNA polymerase extends the 3′ end of each primer along the template strands with a temperature raised back to 70-72 degree Celsius.
All this require a stable RNA Polymerase that can tolerate a high heat and not become denatured most especially in the third step of extension.
Learn more about PCR: https://brainly.com/question/17555761
