When transforming bacterial cells, a researcher is interested in generating bacteria that exogenously express the gene that encodes GFP (green fluorescent protein). After transformation, she finds that no bacteria colonies grew on her selective media. Which of the following best explains the results of her transformation protocol?
a. The concentration of the antibiotics in the selection media was too low, therefore no bacteria grew.
b. Her bacterial cells were not competent so they took up excessive amounts of the GFP-containing plasmid.
d. She forgot the heat-shock step, so the DNA was not forced into bacterial cells.
d. The plasmid that she used contained a sequence that encoded antibacterial resistance.

The following best explains the results of her transformation protocol - C. She forgot the heat-shock step, so the DNA was not forced into the bacterial cells.

Green fluorescent protein

a protein made up of 238 amino acid molecules and it turns bright green color when blue or ultraviolet light falls on it. GFP without any cofactors and gene products form the internal chromophore and is used as a reporter for the expression of several genes, used for making biosensors. GFP can be easily introduced and maintained by using transgenic techniques, in bacteria, yeasts, fungi, fish, and mammals.

Transformation

It is a process in which bacteria take up the foreign desired DNA by restriction digest and ligation process used in DNA cloning. The heat-shock step is used for the insertion of the constructed plasmid to insert into bacterial cells.

Learn more about the heat-shock step:

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