Which of the following does not regulate gene expression after transcription?

Alternative splicing
Transcription factor binding
Translational repressor proteins
Modifications to the 5′ cap
MicroRNA

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transcription factor binding

The mechanism that does not regulate gene expression after transcription is 'Transcription factor binding'. Transcription factors are factors that control gene expression before transcription.

During transcription, a fragment of DNA (e.g., a gene sequence) is used as template to create an RNA, usually, an mRNA that is then used as a template to create a protein by a process called translation.

Transcription factors are DNA binding proteins involved in the transcription of DNA into RNA.

These proteins (transcription factors) bind to promoter and enhancer regions of DNA and thus activate or repress transcriptional activity (i.e., gene expression)

In conclusion, the mechanism that does not regulate gene expression after transcription is 'Transcription factor binding'. Transcription factors are factors that control gene expression before transcription.

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