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A student prepares a cell homogenate from spinach leaves. She performs a cell fractionation experiment to determine the shortest amount time of centrifugation at 2000xg that will yield the highest percentage of chloroplasts in the pellet fraction. She splits the spinach homogenate into 5 equal parts and centrifuges the parts at 2000xg for 0, 5, 10, 15 or 20 min. She then determines the proportion of chloroplasts in the supernatant and pellet fractions. The controlled variable(s) in the student's experiment is/are:_____.
a. proportion of chloroplasts in each fraction.
b. use of same plant homogenate throughout.
c. time of centrifugation in minutes.
d. g-force of centrifugation.

Respuesta :

samueladesida43

Answer:

b. use of same plant homogenate throughout.

Explanation:

In an experiment, the controlled variable, also called the CONSTANT is the variable that is kept the same throughout the course of the experiment in order not to influence the result or outcome of the experiment.

In this question, a student performs a cell fractionation experiment to determine the shortest amount time of centrifugation (manipulated variable) at 2000xg that will yield the highest percentage of chloroplasts (responding variable) in the pellet fraction. In this experiment, the variable being controlled is same spinach plant homogenate used throughout.

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