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Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymerase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus).

Taq polymerase is a common enzyme used for PCR amplification. It is similar to E. coli DNA dependant DNA polymerase I. It is so because of the strong homology at the amino acid level. The 3′-OH nucleotide addition site, the dNTP/DNA binding sites, and the 5′-3′ exonuclease sites of both the enzymes.

What is E. coli DNA dependant DNA polymerase I?

The DNA polymerase I is mainly used for repair of the damaged DNA. It also helps to connect Okazaki fragments by deleting RNA primers.

E. coli DNA dependant DNA polymerase I has similar properties like Taq polymerase as they both have strong homology at the amino acid level.

The 3′-OH nucleotide addition site, the dNTP/DNA binding sites, and the 5′-3′ exonuclease sites of both the enzymes.

Thus, it can be concluded that E. coli DNA dependant DNA polymerase I is the enzyme similar to Taq polymerase.

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