Respuesta :
I need 0.1g of protein
HOW TO CALCULATE NUMBER OF MOLES?
- The number of moles of a substance in a solution can be calculated by multiplying the volume by the molarity of the solution i.e.
no of moles (n) = Molarity (M) × Volume (V)
In this question, 100 ml of a 20 uM solution of a protein was given.
Since 1uM = 10^-6M
20uM = 20 × 10-⁶
= 2 × 10-⁵M
1L = 1000ml
100mL = 100/1000
= 0.1L
no of moles = 2 × 10-⁵M × 0.1L
no of moles = 2 × 10-⁶mol
HOW TO CALCULATE MASS:
- Mass of a substance can be calculated by multiplying the molar mass and the number of moles i.e.
Mass = moles × Molar mass
Molecular mass of protein = 50,000g/mol
Mass = 2 × 10-⁶mol × 50000g/mol
Mass = 0.1g
Therefore, 0.1gram of protein is needed
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You will need 0.1 g of the protein.
Molarity of a solution is simply defined as the mole of solute per unit litre of water.
Mathematically, it is expressed as:
[tex]Molaity = \frac{Mole }{Volume }[/tex]
With the above information in mind, we can determine the amount of the protein needed as follow:
Step 1:
Data obtained from the question
Volume = 100 mL
Molarity = 20 μM = 20×10¯⁶ M
Molecular weight of protein = 50000 g/mol
Mass of protein=?
Step 2
Conversion of 100 mL to litre (L).
1000 mL = 1 L
Therefore,
[tex]100 mL = \frac{100 mL * 1 L}{100 mL }\\\\100 mL = 0.1 L[/tex]
Step 3
Determination of the mole of the protein
Volume = 0.1 L
Molarity = 20×10¯⁶ M
Mole of protein =?
Mole = Molarity × Volume
Mole of protein = 20×10¯⁶ × 0.1
Mole of protein = 2×10¯⁶ mole
Step 4
Determination of the mass of the protein
Mole of protein = 2×10¯⁶ mole
Molecular weight of protein = 50000 g/mol
Mass of protein =?
Mass = mole × molar mass
Mass of protein = 2×10¯⁶ × 50000
Mass of protein = 0.1 g
Therefore, the amount of the protein needed is 0.1 g
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