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I need 0.1g of protein

HOW TO CALCULATE NUMBER OF MOLES?

  • The number of moles of a substance in a solution can be calculated by multiplying the volume by the molarity of the solution i.e.

no of moles (n) = Molarity (M) × Volume (V)

In this question, 100 ml of a 20 uM solution of a protein was given.

Since 1uM = 10^-6M

20uM = 20 × 10-⁶

= 2 × 10-⁵M

1L = 1000ml

100mL = 100/1000

= 0.1L

no of moles = 2 × 10-⁵M × 0.1L

no of moles = 2 × 10-⁶mol

HOW TO CALCULATE MASS:

  • Mass of a substance can be calculated by multiplying the molar mass and the number of moles i.e.

Mass = moles × Molar mass

Molecular mass of protein = 50,000g/mol

Mass = 2 × 10-⁶mol × 50000g/mol

Mass = 0.1g

Therefore, 0.1gram of protein is needed

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You will need 0.1 g of the protein.

Molarity of a solution is simply defined as the mole of solute per unit litre of water.

Mathematically, it is expressed as:

[tex]Molaity = \frac{Mole }{Volume }[/tex]

With the above information in mind, we can determine the amount of the protein needed as follow:

Step 1:

Data obtained from the question

Volume = 100 mL

Molarity = 20 μM = 20×10¯⁶ M

Molecular weight of protein = 50000 g/mol

Mass of protein=?

Step 2

Conversion of 100 mL to litre (L).

1000 mL = 1 L

Therefore,

[tex]100 mL = \frac{100 mL * 1 L}{100 mL }\\\\100 mL = 0.1 L[/tex]

Step 3

Determination of the mole of the protein

Volume = 0.1 L

Molarity = 20×10¯⁶ M

Mole of protein =?

Mole = Molarity × Volume

Mole of protein = 20×10¯⁶ × 0.1

Mole of protein = 2×10¯⁶ mole

Step 4

Determination of the mass of the protein

Mole of protein = 2×10¯⁶ mole

Molecular weight of protein = 50000 g/mol

Mass of protein =?

Mass = mole × molar mass

Mass of protein =  2×10¯⁶ × 50000

Mass of protein = 0.1 g

Therefore, the amount of the protein needed is 0.1 g

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