Determine the concentration of the sugar solution in the graduated cylinder using the following equation:
% m/V=Mass in Plastic Cylinder (g)/ Total Volume (mL)×100%

Record the concentration of the sugar solution in three places: In Data Table 8, for “Concentration,” in Data Table 9 for “Final Concentration” in the “Solution 0” row, and in Data Table 9 under “Initial Concentration” in the “Solution 1” row.
Tare the scale by pressing the Φ/T button so that the scale reads 0.0 g.
Place the graduated cylinder containing the solution on the scale.
Subtract the mass of the dry, empty graduated cylinder from the mass of the cylinder containing the solution. The difference is the mass of the sugar solution.
Record the mass of the sugar solution in Data Table 9 for “Solution 0.”
Calculate the density of the sugar solution and record it in Data Table 9 for “Solution 0.”
Pour the sugar solution (Solution 0) into the dry, empty 100 mL glass beaker.
Rinse the graduated cylinder well with distilled water.
Use the serological (graduated) pipet with bulb to transfer 2.5 mL of the sugar solution from Solution 0 (which is in the glass beaker) into the graduated cylinder.
Add approximately 15 mL of distilled water to the plastic cylinder containing Solution 0.
Swirl the graduated cylinder to thoroughly mix the sugar solution with the distilled water. If necessary, use the serological pipet as a stirring rod to thoroughly mix the sugar solution. Rinse the pipet with distilled water after mixing.
Using the short stemmed pipet, continue adding distilled water to the 25 mL mark of the cylinder.
Tare the scale by pressing the Φ/T button so that the scale reads 0.0 g and then place the graduated cylinder containing the solution on the scale.
Subtract the mass of the dry, empty graduated cylinder from the mass of the cylinder containing the solution.
Record the mass of the new sugar solution in Data Table 9 for “Solution 1.”
Calculate the density of the sugar solution and record in Data Table 9 for “Solution 1.”
Determine the Final Concentration (% m/V) for Solution 1 using the following equation:
C1×V1=C2 ×V2
In this equation:
C1=Initial Concentration
V1=Volume Transferred
V2=25 mL
C2= Final Concentration

Record the Final Concentration of “Solution 1” in Data Table 9 under “Final Concentration.”
Pour Solution 0 from the glass beaker into the sink and thoroughly dry the beaker.
Transfer Solution 1 from the graduated cylinder to the dry, empty glass beaker.
Repeat steps 19 through 31 for the remaining 3 dilutions (4.5 mL, 3.0 mL, and 6.0 mL) in Data Table 9.
Note: In step 20, transfer the appropriate volume of sugar solutions (4.5 mL, 3.0 mL, and 6.0 mL). Also, use the diluted solution from the previous step as the initial concentration on the next dilution. Do not use the same initial concentration of 32% for all the dilutions.