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DNA sequencing using the Sanger method requires ________.
A) template, primer, DNA polymerase, dNTPs, ddNTPs.
B) template, primer, DNA polymerase, rRNA, dNTPs, ddNTPs.
C) template, primer, DNA polymerase, mRNA, dNTPs.
D) template, primer, DNA polymerase, mRNA, dNTPs, ddNTPs.
E) none of the above.

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Answer:

Sanger sequencing requires a DNA template, a sequencing primer, a thermostable DNA polymerase, nucleotides (dNTPs), dideoxynucleotides (ddNTPs), and buffer. Thermal cycling in the sequencing reactions amplifies extension products that are terminated by one of the four ddNTPs.

Explanation:

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