Another major problem that was associated with Miller–Urey experiment was that scientist claimed that reactive mixture of methane and ammonia used doesn't did exist in large amounts on early Earth.
The Miller–Urey experiment serves as a chemical experiment that show that amino acid can be produced by mixture of gases and water.
Therefore, the mixture of gases requires cannot be gotten on earth which is a major challenge.
Learn more about Miller–Urey experiment at;
https://brainly.com/question/25870256