During the course of a catalytic reaction the following occurs: 1) Substrate is covalently bound and oriented with proximity to the active site residues. 2) Negative charge builds up on the substrate and is stabilized. 3) Oxidation of the enzyme followed by reduction to complete the catalytic cycle. What type of chemical species can facilitate these reactions? A) nucleophilic amino acids B) electrophilic groups C) prosthetic phosphate groups D) transition metal anions E) transition metal cations