Correlation between hemolytic activity, cytotoxicity and systemic in vivo toxicity of synthetic antimicrobial peptides

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21-07-2022
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Correlation between hemolytic activity, cytotoxicity and systemic in vivo toxicity of synthetic antimicrobial peptides

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tutorconsortium344 tutorconsortium344 · Answer 1 · 2022-07-31T07:29:03+02:00

Correlation between hemolytic activity, cytotoxicity and systemic in vivo toxicity of synthetic antimicrobial peptides are described below.

What is hemolytic activity?

A bacterium's or molecule's hemolytic activity refers to its capacity to break down red blood cells and release haemoglobin as a result. For instance, bacteria are cultivated on blood agar plates to test this capability. When hemolysis is present, the bacteria will group into colonies that are encircled by a clear zone.

In the early stages of the development of antimicrobial peptides toward therapeutic applications, the use of non-standard toxicity models is a barrier. A library of 24 peptide-based antimicrobials with narrow spectrum action against veterinary infections underwent a thorough in vitro and in vivo toxicity evaluation, which is described here. The compounds' hemolytic activity was assessed against four distinct species, with canine erythrocytes showing the highest relative sensitivity to the compounds, rat and human cells showing intermediate sensitivity, and bovine cells exhibiting the lowest sensitivity.

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