In electron transport chain (ETC) , Complex IV, also known as cytochrome c oxidase, oxidises cytochrome c and delivers the electrons to oxygen, the final electron carrier in mitochondria.
The electrons in the electron transport chain (ETC) pass through a chain of proteins that raises its reduction potential and results in an energy release. A proton gradient is produced by pumping hydrogen ions (H+) from the mitochondrial matrix to the intermembrane space, with the majority of this energy being lost as heat in mitochondria .
This gradient generates an electrical difference with a positive charge outside and a negative charge inside, increasing the acidity.
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