Depurination is a spontaneous chemical processes that is known to cause the most severe DNA damage in cells. DNA can be depurinated to liberate guanine and adenine.
- The DNA molecule either has the typical sequence or lacks one nucleotide pair.
- A daughter DNA molecule can be produced during the replication of a double-stranded DNA molecule that has experienced depurination on an A or G nucleotide on one of the strands, depending on whether the sequence is normal or not.
- The replication machinery can simply skip over the depurinated base and go to the following nucleotide on the strand that contains the damaged nucleotide, generating a daughter DNA molecule that is missing that entire nucleotide pair.
- Since the sequence is unaltered on the other DNA strand, a normal, complementary daughter strand is created during replication, resulting in a double-stranded DNA with all of its typical properties. It's crucial to keep in mind that a cell's regular DNA machinery.
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