There are several methods of gene editing, including CRISPR-Cas9 the most widely used method.
One of the most popular methods of gene editing currently is the use of CRISPR/Cas9.Genome editing relies on the production of site-specific double-strand DNA breaks (DSBs) and the subsequent endogenous repair through the error-prone non-homologous end-joining (NHEJ) or the error-free homology-directed repair (HDR) pathways.
CRISPR/Cas9 is a technique that allows for the highly specific and rapid modification of DNA in a genome, the complete set of genetic instructions in an organism. In comparison to other technologies CRISPR/Cas9 is reliable Due to the RNA-based nature of the system, CRISPR is the most flexible, scalable, and user-friendly of the gene-editing platforms.
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