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If after gram-staining a pure bacterial culture red and purple cocci are observed and the adjacent cells were not of the same colour, It means it's an old stock of culture.
The Gram stain process is a multi-step differential staining procedure. Differential staining separates organisms based on how they respond to various stains. To put it another way, two organisms in a sample that has been stained differently might appear to be different colours.
Gram-positive and Gram-negative are the two primary subtypes of bacteria. Based on how these bacteria respond to the Gram stain, several types are identified. The hue of a Gram stain is purple. The bacteria in a sample will either stay purple or change pink or red when the stain and bacteria interact.
The bacteria are Gram-positive if they continue to be purple.
The bacteria are Gram-negative if they turn pink or crimson.
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