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What is the process of dideoxy sequence?
Sanger sequencing mimics the natural process of DNA replication by using dummy nucleotides to stop replication whenever a specific nucleotide is encountered. Because this truncated replication happens repeatedly, nucleic acids of various lengths accumulate and can be used to determine the position of each nucleotide in the sequence
ddNTPs are included (dideoxynucleoted)
A representation of 16 nucleotides arranged in a single horizontal strand of DNA is shown. Horizontal cylinders represent the deoxyribose sugar molecule and vertical rectangles represent the nitrogenous base in each nucleotide. The deoxyribose sugar and nitrogenous base of a single DNA strand are shown in grey. Below the single strand are four DDNTPs and four individual nucleotides. Sugar molecules are black in the bottom four DDNTPs in the bottom four individual nucleotides. To represent the bases' chemical identities, the nitrogenous bases on the DDNTPs and the lower four nucleotides are colored differently: DDATP is green, DDCTP is orange, DDTTP is red, and DDGTP is blue. Thymine is blue, cytosine is orange, and adenine is green.
DNA sequence :
DNA sequencing is the process of determining the order of the nucleotides within a gene. The first DNA sequencing methods were time-consuming, but a major breakthrough came in 1975 with the development of the Sanger sequencing method. Sanger sequencing is named after the English biochemist Frederick Sanger, and it is also known as chain-termination sequencing or dideoxy sequence. The Sanger method was used to sequence the human genome some 25 years after its invention, and it remains an important method in laboratories around the world today, despite many technological advances and modifications.
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