In E . coli, to repair a thymine dimer by nucleotide excision repair, the sequential actions of enzymes is in order: nuclease, DNA polymerase, DNA ligase .
The process of nucleotide excision repair is controlled in Escherichia coli by the UvrABC endonuclease enzyme complex.
After scans and recognition of distorted helices by UvrA-UvrB complex, UvrC forms a UvrBC dimer which causes cleavage of phosphodiester bond 4 nucleotides.
Thus, DNA polymerases delta or epsilon are used to resynthesize the bases removed during repair of pyrimidine dimers and ligase to bind the fragments.
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