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The discharge of phosphate in detergents to the environment has led to imbalances in the life cycle of freshwater lakes. A chemist uses a spectrometric method to measure total phosphate and obtains the following data for known standards: (a) Draw a curve of absorbance vs. phosphate concentration.

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What is spectrometric method?

  • A technique called spectrophotometry uses light intensity measurements as a beam of light travels through a sample solution to determine how much a chemical compound absorbs light.
  • Every chemical either absorbs or transmits light across a specific spectrum of wavelengths, according to the fundamental principle.
  • There are two main techniques used among the various forms of spectrophotometry:
  • ultraviolet-visible range spectrophotometry, which examines the reflectance of certain spectra,
  • and absorption spectrophotometry, which examines the absorption of radiation and particular spectra of light.
  • Applications of spectrophotometry are useful for determining how well gases, liquids, and solids transmit, reflect, and absorb light.

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