When organisms perform normal metabolic processes, hydrogen peroxide is often created as a byproduct. Hydrogen peroxide can
be harmful to cells, however, so organelles known as peroxisomes release an enzyme known as catalase to break down hydrogen
peroxide into water and oxygen.
2 H₂O₂+ catalase - O₂ + 2H₂O
Emily wanted to perform an experiment to test how temperature affects the action of catalase. To do this, she obtained four identical
test tubes and poured 5 mL of hydrogen peroxide into each tube. Then, she placed the tubes into separate incubators set at 5°C,
25°C, 35°C, and 45°C. She allowed the tubes to equilibrate at their respective temperatures for 20 minutes and then added exactly
1 ml of cold catalase to each tube. After three minutes, she added 6 mL of sulfuric acid to each tube to stop the reaction. Finally, she
titrated each solution to determine the amount of hydrogen peroxide remaining in each tube.
Which of the following factors was allowed to change during Emily's experiment?
00000
the amount of sulfuric acid added to each tube
the temperature of each incubator
the amount of time each tube was allowed to equilibrate
the amount of hydrogen peroxide remaining in each tube
the amount of catalase added to each tube
Can be more than one answer

Peroxisomes are organelles located in the cytoplasm of eukaryotic cells responsible for the oxidation and elimination of hydrogen peroxide as in the experiment. These also have functions related to lipid metabolism and converting reactive oxygen species.

The formation of peroxisomes occurs by division of existing peroxisomes or by generation of new peroxisomes from the endoplasmic reticulum and mitochondria.

As for the experiment, we see that factors such as the amount of sulfuric acid added, the time in which each tube is equilibrated, the amount of hydrogen peroxide in each tube and the amount of catalase added are measured and do not change during the experiment while emily does give a space for the temperature of the tubes to change at their own mercy, this being a variable that is not measured.

Therefore, we can confirm that the factor that was allowed to change during Emily's experiment was the temperature of each incubator.

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