In the cell cycle, sister chromatids first make up one chromosome in the prophase stage of mitosis and meiosis.
Cell cycle is the life cycle of eukaryotic cells from a quiescent beginning, growth, duplication of DNA followed by mitosis and division.
The cell cycle is made up of mitotic cell division i.e. division of cell's nucleus, which in turn is made up of stages as follows:
Therefore, in the cell cycle, sister chromatids first make up one chromosome in the prophase stage of mitosis and meiosis.
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