The correct option is (A) your smear was not made from a pure culture
If some cells in your gram stain are red/pink rods and some are purple cocci, your smear was not made from a pure culture
Bacterial identification is assisted by a Gram stain test. Blood, urine, or cerebrospinal fluid are examples of bodily fluids from which the test sample can be drawn but which ordinarily do not contain bacteria. Additionally, a sample may be obtained from the throat, lungs, genitalia, or skin, or another suspected infection location.
Gram-positive bacteria show up purple-blue under a microscope because their thick peptidoglycan membrane can hold the dye. The bacteria is known as gram-positive since the test result was positive. Pink-red stains are left by gram-negative bacteria. Since their peptidoglycan layer is thinner, it loses its blue hue.
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