Gram-negative cells may appear to be gram-positive if you decolorize a slide for an excessively long time.
Not longer than 15 seconds should the decolorizer be left on the slide. Even gram-positive cells lose their crystal violet appearance and begin to stain red if the decolorizer is left on for an extended period of time. Here is how you stain something.
The most important part of the process is right now. Gram-positive bacteria will appear Gram-negative if the decolorizer is left on for too long. Gram-negative bacteria will appear to be Gram-positive if the decolorizer is not left on for long enough.
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