is an experimental technique that is used to separate proteins based on their ability to bind to a specific ligand. a) sds-page gel electrophoresis b) ion-exchange chromatography c) size-exclusion chromatography d) affinity chromatography e) none of the above

Respuesta :

Proteins can be separated experimentally based on how well they bind to particular ligands using ion-exchange chromatography.

What method is available to sort proteins based on variations in net charge?

pH. Depending on pH, total protein charge affects protein stability and ion exchange media binding. Proteins, peptides, amino acids, and nucleotides are examples of charged biological molecules that can be separated using ion exchange chromatography.

What methods are used for protein separation?

Ion-exchange chromatography, affinity chromatography, dialysis, ultrafiltration, size-exclusion chromatography, and electrophoresis (including SDS-PAGE, isoelectric focusing, and capillary electrophoresis) are a few of the frequently used methods for separating proteins.

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