3. assuming a circular piece of dna (a plasmid) was used as a starting material, how many restriction sites were there in the dna sample in lane 4? now assume the plasmid used as a starting material was linearized. how many restriction sites were there in the dna sample in lane 6?

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21-11-2022
Biology

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3. assuming a circular piece of dna (a plasmid) was used as a starting material, how many restriction sites were there in the dna sample in lane 4? now assume the plasmid used as a starting material was linearized. how many restriction sites were there in the dna sample in lane 6?

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vtraviparihar vtraviparihar · Answer 1 · 2022-11-24T08:13:42+01:00

Assuming a circular piece of DNA (a plasmid) was used as a starting material, how many restriction sites were there in the DNA sample in lane 4 and now assume the plasmid used as a starting material was linearity. the number of restriction sites were there in the DNA sample in lane 6 are 2 restriction sites.

What is a plasmid ?

It is the small circular piece of DNA which contains the genetic material for the inheritance and the genetic information is stored in it.

A small linear piece of DNA that is present in the circular form is linearized with the help of enzymes and running them on gels then keeping them at higher temperature in order to check the heat inactivation.

Lane 6 is the smallest lane having DNA pieces where the circular DNA piece will have three restriction sites and the linear ones have two restriction sites.

Learn more about linear DNA at :

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