Tandem affinity purification type of fusion tag would you introduce to your protein to purify both the protein you have been studying and the proteins interacting with it for determining what other proteins might be interacting with your studying.
Tandem affinity purification, or TAP, enables the swift and sterile separation of antagonized proteins and their interacting partners from cell lysates. The TAP method was initially created for yeast, but it has since been modified for use with other cells and organisms. Regardless of scale, protein purification requires methods that are extremely specific and reliable. Since tagging your protein of interest can make the purification process simpler, increase the yield and solubility of your protein of interest, and encourage proper folding of their fusion partners, it can be very helpful.
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