The original concentration in the sample of volume 10⁻⁶ mL is
Concentration:
The quantity of solute present in a given quantity of solution is known as the concentration of a substance. Molarity is usually used to express , the extent of concentration. Molarity is defined as the number of moles of solute in 1 L of solution.
Number of colonies in the plate = 259 colonies
Volume of the sample = 10⁻⁶ mL
Original concentration = (Number of colonies in the plate) / (Volume of the sample)
= 259 / 10⁻⁶ = 2.59 × 10⁸ cfu / mL
Hence, the original concentration of the solution is 2.59 × 10⁸ cfu / mL.
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