Ambiguity is burnt away and the truth is momentarily discovered to the audience as Claudius confesses his guilt (Hamlet) to them.
Hamlet has been told the reality via the ghost. Claudius is in reality accountable for the murder of his Father, and on this speech, we see how deep his guilt and angst go.
thru Claudius' soliloquy, Shakespeare famous Claudius's inner man or woman and similarly characterizes his disposition, even though the regret he feels isn't always for his slain brother but for the consequences he faces due to it. Shakespeare is able to depict Claudius's internal struggle and the way it reflects his individuality.
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