looking at the cell of a photosynthetic microorganism, you observe that the chloroplasts are green in brightfield microscopy and red in fluorescence microscopy. You conclude that
a. chlorophyll is fluorescent b. the magnification has distorted the image c. you're not looking at the same structure in both microscopes d. the stain masked the green color e. none of the above

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24-11-2022
Biology

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looking at the cell of a photosynthetic microorganism, you observe that the chloroplasts are green in brightfield microscopy and red in fluorescence microscopy. You conclude that
a. chlorophyll is fluorescent b. the magnification has distorted the image c. you're not looking at the same structure in both microscopes d. the stain masked the green color e. none of the above

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RupaBera RupaBera · Answer 1 · 2022-11-26T10:19:49+01:00

As chlorophyll is fluorescent, you can see that the chloroplasts are red in fluorescence microscopy and green in brightfield microscopy.

What shade fluoresces in chlorophyll?

When exposed by light of around 400-700 nm (photosynthetically active radiation, or PAR), photosynthetic tissues and organisms emit red to far-red light, which is known as fluorescence (McCree 1972). In this spectrum, red and blue light are more effective at stimulating chlorophyll than green light.

What is a study of chlorophyll fluorescence?

Chlorophyll fluorescence is a frequent method in plant physiology for measuring photosystem II (PSII) activity since it is non-invasive. One of the most effective and popular methods for examining how pressures affect the photosynthetic process is chlorophyll fluorescence analysis.

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