They have different rRNA.
A lipopolysaccharide-containing outer membrane surrounds the peptidoglycan cell wall that encases gram-negative bacteria. In contrast to Gram-negative bacteria, which have an outer membrane, gram-positive bacteria have layers of peptidoglycan that are many times thicker.
The crystal violet stain used in the Gram staining method of bacterial differentiation is not retained by gram-negative bacteria.
There are gram-negative bacteria in almost every place on Earth that supports life. Escherichia coli, a model organism, and other pathogenic bacteria, including Pseudomonas aeruginosa, Chlamydia trachomatis, and Yersinia pestis, are all examples of gram-negative bacteria.
Conjugation is the process of directly transferring genetic material from one bacterium to another.
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