Respuesta :
Correct answer: C). Excess DNA is saved for other testing
The DNA fingerprinting is a method, in which there is the isolation of DNA from the blood sample and then identify variable regions in the DNA sequence, which are repeating many times also called as minisatellites.
Steps of DNA Fingerprinting:
- The blood sample is collected and then DNA is extracted from the sample
- The DNA fragment is created by using restriction enzyme
- Then fragment is loaded into gel electrophoresis
- After that, the x-ray film radiography is done to see the sequence.
Hence, option C is not a step in DNA fingerprinting.
Excess DNA is saved for other testing is not the step of the DNA fingerprinting.
Further Explanation:
DNA (Deoxyribose nucleic acid) fingerprinting is the molecular genetics method that helps in the identification of individuals. The DNA can be extracted from hair, blood, and other fluids. It is also known as an identity test. This method is discovered by Alec Jeffrey’s in 1984. This technique is based on the highly variable base pair sequence on DNA that is unique to each individual.
DNA fingerprinting includes the following steps:
- The first step is collecting the sample that contains cells such as hair, blood, and skin.
- DNA is isolated from the cell and purified.
- The DNA strands are cut from particular points by using the restriction enzymes. The enzymes help in forming fragments of variable length.
- These fragments are separated by using the gel electrophoresis method. The shorter fragment moves faster than a longer fragment.
- The separated fragments than subjected to the blotting in which they split into single strands and transferred into a nylon sheet.
- After that, autoradiography is performed in which the DNA strands come in contact with the DNA probes.
- The sheet is then exposed to the X-rays that produce dark marks in the region where the DNA probe is present. The resultant pattern can be analyzed.
Learn More:
- Learn more about meiosis https://brainly.com/question/1600165
- Learn more about the process of molecular diffusion in a cell https://brainly.com/question/1600165
- Learn more about human sperm and egg cell https://brainly.com/question/1626319
Answer Details:
Grade: High School
Subjects: Biology
Topic: DNA Fingerprinting
Keywords:
Deoxyribose nucleic acid, molecular, genetic, identification, blood, hair, variable, fingerprinting, restriction, enzyme, electrophoresis, nylon, blotting, probes.
