Answer:
Therefore, required mass of insert DNA is 30 ng
Explanation:
DNA ligation is the process of joining two DNA fragments together through the action of an enzyme for example DNA ligase. The vector DNA often a plasmid is used to insert a desired DNA sequence into a host cell DNA.
The formula for the calculation of required insert DNA mass is given below:
Required mass insert (ng) = desired insert/vector molar ratio x mass of vector (ng) x ratio of insert to vector lengths
Desired insert/vector molar ratio = 3 : 1
Mass of vector = 30 ng
Ratio of insert vector lengths = 4 : 12 = 1 : 3
Required mass of insert DNA = 3/1 × 30 ng x 1/3 = 30 ng
Therefore, required mass of insert DNA is 30 ng
