during dna replication in e. coli, which of the following enzymes removes the rna nucleotides from the primer and adds equivalent dna nucleotides to the 3' end of okazaki fragments? group of answer choices ligase helicase dna polymerase i dna polymerase iii

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21-11-2022
Biology

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during dna replication in e. coli, which of the following enzymes removes the rna nucleotides from the primer and adds equivalent dna nucleotides to the 3' end of okazaki fragments? group of answer choices ligase helicase dna polymerase i dna polymerase iii

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charithran8975 charithran8975 · Answer 1 · 2022-11-27T15:42:15+01:00

A DNA polymerase I take out the RNA primer and then use DNA to fill in the spaces.

The gaps are filled up with DNA once the RNA primer is removed by DNA polymerase I. The procedure to eliminate the nicks, however, cannot be catalysed by DNA polymerase I. By creating the phosphodiester bond, DNA ligase—another enzyme—seals the nicks, creating a continuous sugar-phosphate backbone for the lagging strand. The Okazaki fragment is synthesised by DNA polymerase I enzyme in prokaryotes up until the preceding RNA primer. The enzyme then performs a simultaneous 5′–3′ exonuclease reaction, eliminating primer ribonucleotides up front and introducing deoxyribonucleotides in their place.

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